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目的:探讨Ⅱ型转化生长因子β受体亲和肽在CCl4诱导的小鼠肝纤维化模型中的抗肝纤维化作用。方法:根据展示肽氨基酸序列合成亲和肽C1;应用CCl4制作小鼠肝纤维化模型;给予亲和肽C1施行干预治疗,同时设立正常对照组、模型组、秋水仙碱组;造模5周后采血并处死小鼠,检测血清中ALT、ALB、TP含量,取肝组织测定羟脯氨酸含量,制作病理组织切片,作HE染色和Masson染色,对肝脏炎症活动度和纤维化程度进行分析。结果:人工合成的Ⅱ型转化生长因子β受体亲和肽C1用于抗肝纤维化治疗能减轻炎症、减少胶原纤维形成,ALT、羟脯氨酸与肝纤维化小鼠模型组相比差异具有统计学意义(P<0.05)。结论:亲和肽C1可以改善实验性肝纤维化小鼠的肝脏组织结构和肝功能,具有较好的抗肝纤维化作用。
Objective: To investigate the anti-hepatic fibrosis effect of type Ⅱ transforming growth factor-β receptor affinity peptide in CCl4-induced murine liver fibrosis model. Methods: Peptide C1 was synthesized according to amino acid sequence of peptide. CCl4 was used to produce mouse hepatic fibrosis model. Peptide C1 was given to the mice for treatment. At the same time, normal control group, model group and colchicine group were established. The mice were sacrificed and the mice were sacrificed. The contents of ALT, ALB and TP in the serum were measured. The content of hydroxyproline in the liver tissue was determined. The pathological sections were made for HE staining and Masson staining to analyze the degree of liver inflammation and fibrosis . Results: The synthetic type Ⅱ transforming growth factor-beta receptor peptide C1 was effective in anti-hepatic fibrosis treatment, which could reduce the inflammation and reduce the formation of collagen fibers. Compared with the model group of ALT, hydroxyproline and hepatic fibrosis Statistically significant (P <0.05). Conclusion: The affinity peptide C1 can improve the hepatic tissue structure and liver function in experimental hepatic fibrosis mice and has a good anti-hepatic fibrosis effect.