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用间接荧光抗体法和2-ME处理法,对117例临床和血清学确诊的乙脑病人血清,进行特异性IgM抗体的检测,试验结果表明:急性期阳性检出率荧光抗体法88.88%,2-ME处理法47.00%,二者相比有非常显著性差异(P<0.01)。根据发病天数前法1～5天的阳性率已高达88.0%,6～32天均维持在90.24～93.55%,而后法的阳性率发病6～10天仅达58.54%,11天以后渐趋下降。37名正常人和29例非乙脑患者,两法均呈阴性。荧光抗体抗核抗体法强阳性患者15例中,有4例出现非特异性反应,但采用正常细胞对照即可排除。
Using indirect fluorescent antibody method and 2-ME treatment method, we detected the serum of 117 patients with clinically and serologically confirmed JE patients. The results showed that the positive rate of antibody was 88.88% in the acute phase, 2-ME treatment 47.00%, there is a very significant difference between the two (P <0.01). According to the number of days before the onset of law 1 to 5 days the positive rate has reached 88.0%, 6 to 32 days maintained at 90.24 ~ 93.55%, while the positive rate of postoperative 6 to 10 days only reached 58.54%, 11 days later decreased . 37 normal subjects and 29 non-E brain patients, both were negative. Among the 15 patients with strong positive anti-nuclear antibody antibody, 4 patients showed nonspecific reaction, but normal cells could be excluded.