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目的检测狂犬病基因工程灭活疫苗(Hep-Flury-dG株)注射犬只后的免疫效力。方法在广州市增城区某村进行狂犬病基因工程灭活疫苗(Hep-Flury-dG株)环境释放试验,登记该村所有家养犬的信息并存档,同时进行疫苗免疫。随机采集免疫前血清样品66份,免疫后21d仍获得血清样品66份,使用ELISA方法检测样品的抗体水平。结果免疫前该村犬只抗体阳转率为26%,免疫后21d抗体阳转率达到83%。经统计分析表明免疫前后抗体水平差异具有统计学意义;性别不是影响抗体水平变化的因素,但年龄可影响抗体水平变化,成犬抗体水平的增加明显高于幼犬。结论狂犬病基因工程灭活疫苗(Hep-Flury-dG株)免疫犬只后可提供足够的保护力,有效保护犬只免受狂犬病病毒感染,因而预防狂犬病从犬-犬或犬-人的传播;为农村地区家养犬建立免疫档案,监控犬只抗体水平,使犬群间形成狂犬病抗体阳性率达到70%以上的坚强免疫带,是预防农村地区狂犬病发生的一种可行、有效的方法。
Objective To detect the immunogenicity of dogs infected with rabies gene inactivated vaccine (Hep-Flury-dG strain). Methods The environmental release test of rabies gene inactivated vaccine (Hep-Flury-dG strain) was conducted in a village in Zengcheng District of Guangzhou City. The information of all domestic dogs in the village was registered and archived. Meanwhile, the vaccine was immunized. 66 serum samples were collected before immunization, and 66 serum samples were obtained 21 days after immunization. The antibody levels of the samples were detected by ELISA. Results Before immunization, the antibody positive rate of the village dogs was 26%. After 21 days of immunization, the antibody positive rate reached 83%. Statistical analysis showed that the antibody levels before and after immunization were statistically significant differences; sex is not a factor affecting the level of antibody changes, but age can affect the changes in antibody levels, adult dogs increased significantly higher antibody levels than puppies. Conclusion The vaccination of rabies engineered inactivated vaccine (Hep-Flury-dG strain) can provide enough protection to protect dogs from rabies virus infection and thus prevent the spread of rabies from dogs and canine dogs and dogs. It is a feasible and effective way to prevent rabies in rural areas by establishing an immunization file for domestic dogs and monitoring the level of antibody in dogs so as to form a strong immunization band with the positive rate of rabies antibody reaching more than 70% among the canines.