论文部分内容阅读
调查和观察了鸭脚树星室木虱在福州糖胶树上的发生和为害特点。同时,测定了鸭脚树星室木虱COⅠ基因部分序列和ITS基因全序列。结果表明,引物对COIF/COIR可扩增到约700 bp的条带,引物对ITSF/ITSR可扩增到约1 000 bp的条带,并对各序列的碱基组成进行了分析。根据COⅠ基因部分序列,利用DNAMAN的Maximum Likelihood方法构建了鸭脚树星室木虱与同翅目其他种类的系统发育树。
Investigation and observation of the duck foot tree star psylla in Fuzhou gum tree occurrence and damage characteristics. At the same time, the partial sequence of COⅠ gene and the ITS sequence of duck psittaci were determined. The results showed that the COIF / COIR primer could amplify to about 700 bp and the ITSF / ITSR of the primer pair could amplify to about 1 000 bp. The base composition of each sequence was analyzed. According to the partial sequence of COⅠ gene, the phylogenetic tree of Pterostilbene spp. And other species of Homoptera was constructed using DNAMAN’s Maximum Likelihood method.