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为揭示西北干燥寒冷环境对机体产生影响的生物学基础,选用ICR小鼠,根据中医病因学说理论,用人工气候箱模拟新疆这种特有的干燥寒冷环境,并作用于小鼠,建立寒燥证证候模型,以正常喂养为空白对照组。试验处理第21天后,用摘眼球取血法和解剖法分别取出小鼠的血液和各种脏器,在HE染色光镜下观察其组织病理学改变,用透射电镜观察其细胞超微结构的改变,并用高效液相色谱-电化学检测法测定小鼠脑组织和外周血中单胺类神经递质的变化。和空白对照组相比,组织形态学方面的改变主要是中心血管的轻度扩张和淋巴细胞增多;超微结构方面主要以水肿表现为主;单胺类神经递质方面,外周血中的去甲肾上腺素(NE)、多巴胺(DA)明显低于对照组(P<0.01),5-羟色胺(5-HT)高于对照组(P<0.05),而脑中去甲肾上腺素、多巴胺和5-羟色胺含量均显著高于对照组(P<0.01)。寒燥环境可导致小鼠体内“寒燥之气”的产生,由此推论寒燥环境是西北寒燥证形成的主要原因;且西北寒燥证不是机体某一脏器的改变,而是整体性、全身性、系统性的改变。
In order to reveal the biological basis of the cold and dry environment affecting the body in northwestern China, ICR mice were selected to simulate the unique dry and cold environment in Xinjiang according to the theory of etiology of Chinese medicine. Syndrome model to normal feeding as a blank control group. Twenty-one days after the experimental treatment, blood and various organs of the mice were taken out by taking blood and dissecting the eyeball respectively, and the histopathological changes were observed under a HE staining light microscope. The ultrastructure of the cells was observed with a transmission electron microscope Changes and determination of monoamine neurotransmitters in brain tissue and peripheral blood of mice by high performance liquid chromatography-electrochemical detection. Compared with the blank control group, the changes in histomorphology were mainly mild dilatation of central blood vessels and lymphocytosis; ultrastructural mainly edema performance; monoamine neurotransmitter, the peripheral blood Norepinephrine (NE), dopamine (DA) were significantly lower than the control group (P <0.01), 5-HT was higher than the control group (P < Serum 5-HT levels were significantly higher than the control group (P <0.01). Cold environment can lead to the body of mice “coldness ” the production, inferring the cold environment is the main reason for the formation of northwest cold syndrome; and cold northwest card is not a change in the organ of an organ, and Is a holistic, systemic, systematic change.