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目的:研究牙移动后牙槽裂隙两侧破骨细胞核因子κB受体活化因子配体(RANKL)和骨保护素(OPG)的表达和作用。方法:选用4只4月龄雄性Beagle犬建立牙槽裂模型,正畸牵引裂隙两侧牙齿向裂隙区移动,实时荧光定量聚合酶链反应(real-time PCR)检测裂隙两侧牙槽骨中RANKL和OPG mRNA的表达水平。结果:正畸牵引16~20周后,牙槽裂间隙缩小以至关闭,原裂隙处有新骨生成。裂隙两侧牙槽骨中RANKL和OPG mRNA表达增加。结论:牙槽裂两侧牙齿受正畸牵引后,RANKL与OPG参与骨重建,裂隙处骨改建活跃。此方法为修复牙槽裂提供新的思路。
OBJECTIVE: To study the expression and role of RANKL and OPG in osteoclasts on both sides of alveolar cleft after tooth movement. Methods: Four 4-month-old male Beagle dogs were used to establish the model of alveolar cleft. The teeth on both sides of orthodontic traction fracture moved to the fracture area. Real-time PCR was used to detect the alveolar bone RANKL and OPG mRNA expression levels. Results: After orthodontic traction for 16 to 20 weeks, the alveolar cleft became smaller and even closed with new bone formation at the original cleft. RANKL and OPG mRNA expression in alveolar bone on both sides of the fracture increased. CONCLUSION: RANKL and OPG are involved in bone remodeling after orthodontic traction on both sides of the alveolar cleft. The bone remodeling is active at the fracture site. This method provides a new way to repair alveolar cleft.