论文部分内容阅读
以脑组织中游离氨基酸含量为指标,观察出生前因氚水照射引起的大鼠脑发育障碍。实验用妊娠11d的大鼠经腹腔单次注入7.4×10~3—3.7×10~6Bq/ml(体水)放射性浓度的氚水。在仔鼠出生后18d将其开颅取出大脑。取一半脑组织用高效液相色谱(HPLC)测定脑组织中游离氨基酸含量。结果表明:受照仔鼠大脑组织中游离氨基酸含量随照射剂量增加而增多。在仔鼠总吸收剂量为0.0038—1.9Gy范围内,大脑组织中游离氨基酸含量增加的百分率与剂量对数值之间可拟合成直线回归方程Y=A+B lgD。
The content of free amino acids in brain tissue was taken as an index to observe the brain development disorder caused by the irradiation of tritiated water before birth. Rats in experiment 11d were injected with tritiated water of 7.4 × 10 ~ 3-7.7 × 10 ~ 6Bq / ml (body water) radioactive concentration through abdominal cavity. The pups were craniotome removed from their brains 18 days after birth. Half of brain tissue was taken for determination of free amino acids in brain tissue by high performance liquid chromatography (HPLC). The results showed that the content of free amino acids in brain tissue of irradiated offspring increased with the increase of irradiation dose. In the range of 0.0038-1.9Gy, the percentage of free amino acid in brain tissue increases with the dose logarithm, and the linear regression equation Y = A + B lgD can be fitted.