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选用Wistar雄性大鼠24只,分为对照组、低氟组、高氟组,每组8只,染氟组分别自由饮用含氟化钠100mg/L、200 mg/L的自来水,对照组自由饮用自来水,喂养20周。取股动脉血离心出血清,采用比色法检测总抗氧化能力(T-AOC)及一氧化氮合酶(NOS)活力,黄嘌呤氧化酶法检测超氧化物歧化酶(SOD)活力,硝酸还原酶法检测一氧化氮(NO)含量。低氟组大鼠血清T-AOC明显高于对照组(P<0.01),而高氟组与对照组相比差异无统计学意义(P>0.05);低氟组NO含量比对照组明显增高,高氟组的NO含量明显高于低氟组(P<0.01)。提示低剂量氟使T-AOC代偿性增加,氟可能通过刺激诱导型一氧化氮合酶(iNOS)异常表达而使NO合成大量增加。
Twenty-four male Wistar rats were randomly divided into control group, low fluoride group and high fluoride group, with 8 rats in each group. Free fluoride, sodium fluoride 100 mg / L and 200 mg / Drink tap water for 20 weeks. Blood samples were taken from the femoral artery and centrifuged to extract the serum. T-AOC and NOS activities were determined by colorimetric assay. The activities of superoxide dismutase (SOD), nitric acid The reductase method was used to detect the content of nitric oxide (NO). The serum T-AOC in low-fluoride group was significantly higher than that in control group (P <0.01), but there was no significant difference between high-fluoride group and control group (P> 0.05) , NO content in high fluoride group was significantly higher than that in low fluoride group (P <0.01). It is suggested that T-AOC may be compensatoryly increased by low dose of fluoride. Fluorine may increase NO synthesis by stimulating the abnormal expression of inducible nitric oxide synthase (iNOS).