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目的探索结核分枝杆菌潜伏感染检测方法。方法取40例结核病人(复苏因子刺激组)和60例PPD阴性正常人(对照组)外周血淋巴细胞于1640培养基中培养,对照不加复苏因子蛋白,复苏因子蛋白质刺激3d,,CCK-8法分析淋巴细胞增殖量。结果复苏因子刺激的结核病人组血淋巴细胞均有不同程度的增殖,增殖率为100%,与对照组比较,差异有统计学意义(P<0.01)。结论外周血淋巴细胞增殖试验可作为结核病的检测指标。
Objective To explore the latent infection detection method of Mycobacterium tuberculosis. Methods Peripheral blood lymphocytes from 40 tuberculosis patients (stimulation factor group) and 60 PPD negative normal people (control group) were cultured in 1640 culture medium. The control group without the apotosis factor and the recovery factor protein stimulated the expression of CCK- 8 method for analysis of lymphocyte proliferation. Results The blood lymphocytes of tuberculosis patients stimulated by resuscitation factor all had different degrees of proliferation with the proliferation rate of 100%. Compared with the control group, the difference was statistically significant (P <0.01). Conclusion Peripheral blood lymphocyte proliferation test can be used as a detection index of tuberculosis.