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高密度脂蛋白(high density lipoprotein,HDL)有抗动脉粥样化的作用,它可能起着从动脉壁细胞带走胆固醇的清除剂作用。最近已报道载脂蛋白A-I·卵磷脂·胆固醇复合物的合成,其物理化学性质类似天然HDL。作者想知道这合成的复合物是否具有HDL的生物学活性,尤其是能否提高从动脉壁细胞中清除胆固醇的速率。他们选用了培养8天的由人类粥样化病变(脂肪条块)的主动脉分离的平滑肌细胞和酶,在原病部位,上述细胞的细胞内脂含量较高,特别是胆固醇酯,而且在培养过程中其脂类水平至少可保留长达12～14天。加入天然HDL和HDL样复合物可显著减低培养细胞的胆固醇水平(从30μg载脂蛋白A-I/
High density lipoprotein (HDL) has anti-atherogenic effects and may act as a scavenger that removes cholesterol from arterial wall cells. The synthesis of apolipoprotein A-lecithin-cholesterol complex has recently been reported, and its physicochemical properties resemble natural HDL. The authors wondered whether this synthetic complex had the biological activity of HDL and, in particular, whether it could increase the rate of cholesterol removal from arterial wall cells. They chose smooth muscle cells and enzymes isolated from the aorta of human atherogenic lesions (fatty streaks) for 8 days. At the site of the disease they had higher intracellular lipid content, especially cholesteryl esters, and in culture The lipid level in the process can be retained for at least 12 to 14 days. Addition of native HDL and HDL-like complexes significantly reduced cholesterol levels in cultured cells (from 30 μg apolipoprotein A-I /