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为考察A3α-肽聚糖(Peptidoglycan,PG)在凡纳滨对虾(Litopenaeus vannamei)的养成期对其免疫酶活性的影响,设立了连续投喂、间隔投喂和浸浴共6个实验组。连续投喂组中PG的添加量分别为0(对照)、0.01%、0.05%和0.1%;间隔投喂组中PG的添加量为0.05%;浸浴组的给予方式为50mg/ml,3h/次/15d。分别在30、60和90d时,测定对虾体内及血浆上清中的酸性磷酸酶(Acid phosphatase,ACP)和碱性磷酸酶(Alkaline phosphatase,AKP)活性,另外,在60d和90d时,测定对虾血细胞内酚氧化酶(Phenoloxidase,PO)活性。结果表明,30d时,PG浸浴组、0.05%和0.1%PG添加量组的ACP活性,各PG作用组的AKP活性均较对照组高,且0.1%PG添加量组的ACP活性及0.05%和0.1%PG添加量组的AKP活性较对照组增长显著(P<0.05);60d时,除间隔投喂组外,各组的ACP活性均较对照组高,且0.1%PG添加量组增长显著(P<0.05),而各实验组的AKP活性均较对照组高,且0.05%PG添加量组增长显著(P<0.05),除浸浴组外,各组血细胞内PO活性均有提高;90d时,浸浴组和间隔组的ACP和AKP活性均较对照组高,且间隔投喂组的AKP活性增长显著(P<0.05);间隔投喂组的血细胞内PO活性较对照组有增长。实验证明,PG能激活对虾的免疫力,并可作为免疫增强剂应用于对虾的生产。
In order to investigate the effect of the production of A3α-peptidoglycan (PG) on the immunoenzyme activity of Litopenaeus vannamei during the growth phase, 6 experimental groups (continuous feeding, intermittent feeding and bathing) . The feeding amount of PG in the continuous feeding group was 0 (control), 0.01%, 0.05% and 0.1%, respectively. The PG dosage was 0.05% in the intermittent feeding group and 50 mg / ml in the immersion group, / Times / 15d. At 30, 60 and 90 days, the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) in shrimp body and plasma supernatant were measured. At 60 days and 90 days, Phenoloxidase (PO) activity in blood cells. The results showed that ACP activity of PG immersion group, 0.05% and 0.1% PG addition group and AKP activity of each PG treatment group were higher than that of control group at 30 d, and ACP activity and 0.05% (P <0.05). Compared with the control group, the activities of AKP in the groups supplemented with 0.1% PG increased significantly (P <0.05). At 60 days, the activities of ACP in each group were higher than those in the control group (P <0.05). However, AKP activity in each experimental group was higher than that in control group, and 0.05% PG addition group had a significant increase (P <0.05). In addition to the immersion group, the intracellular PO activity was increased ; At 90 days, ACP and AKP activities in bath group and septum group were higher than those in control group, and the AKP activity in interval fed group increased significantly (P <0.05). The intracellular PO activity in interval fed group was higher than that in control group increase. Experiments show that PG can activate shrimp immunity, and can be used as immune enhancer shrimp production.