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目的观察腺病毒介导的自杀基因对消化系肿瘤的杀伤作用。方法腺病毒介导的自杀基因HSV-TK分别转染结肠癌LOVO细胞、胃癌MGL-803细胞和肝癌BEL-7402细胞,比较腺病毒载体对不同肿瘤细胞的转染效率。加入前药丙氧鸟苷(GCV),通过MTT法检测细胞存活率,观察单纯疱疹病毒1型胸苷激酶/丙氧鸟苷(HSV-TK/GCV)系统对不同肿瘤细胞的杀伤作用和旁观者效应。结果在GCV浓度100mg/L以上时,3种转基因肿瘤细胞均可被HSV-TK/GCV系统完全杀伤;旁观者效应以MGL-803细胞最为明显,与LOVO细胞和BEL-7402细胞相比,差异有显著性意义(P<0.05);腺病毒对肿瘤细胞的转染效率强弱依次为BEL-7402细胞、MGL-803细胞和LOVO细胞。结论重组腺病毒可以作为消化系肿瘤基因治疗的高效载体。HSV-TK/GCV系统对胃癌MGL-803细胞的作用最佳。
Objective To observe the killing effect of adenovirus-mediated suicide gene on the digestive system tumors. Methods Adenovirus-mediated suicide gene HSV-TK was transfected into colon cancer LOVO cells, gastric cancer MGL-803 cells and hepatocellular carcinoma BEL-7402 cells respectively. The transfection efficiency of adenoviral vector on different tumor cells was compared. The prodrug ganciclovir (GCV) was added and cell viability was measured by MTT assay to observe the killing effect and bystanderness of HSV-TK / GCV system on different tumor cells Effect. Results All of the three kinds of transgenic tumor cells were completely killed by HSV-TK / GCV system when GCV concentration was above 100mg / L. Bystander effect was the most obvious in MGL-803 cells. Compared with LOVO cells and BEL-7402 cells, (P <0.05). The transfection efficiencies of adenovirus on tumor cells were BEL-7402 cells, MGL-803 cells and LOVO cells in turn. Conclusion Recombinant adenovirus can be used as an efficient vector for gene therapy of digestive system tumors. HSV-TK / GCV system has the best effect on gastric cancer MGL-803 cells.