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目的探讨汉黄芩素对胶质瘤U87细胞增殖、侵袭的影响及其相关机制。方法 U87细胞随机分为对照组(加入20μL的培养液)、(0、50、100)μmol/L汉黄芩素处理组;细胞培养48h,应用MTT法检测细胞增殖情况,通过TranswellTM侵袭实验检测细胞侵袭能力,荧光实时定量PCR测定ezrin mRNA表达,Western blot法检测ezrin、Bcl-2、Bax蛋白表达及ezrin蛋白磷酸化水平,用末端脱氧核糖核酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法(TUNEL)测定肿瘤细胞凋亡指数(AI)。结果与0μmol/L汉黄芩素组以及对照组相比,(50、100)μmol/L汉黄芩素组细胞增殖抑制率增加,且100μmol/L汉黄芩素组细胞增殖抑制率明显高于50μmol/L汉黄芩素组。随着汉黄芩素浓度的增加,平均穿膜细胞数以及ezrin mRNA、ezrin蛋白、ezrin蛋白磷酸化水平、Bcl-2蛋白表达水平逐渐降低,而Bax蛋白表达水平、AI逐渐增加;0μmol/L汉黄芩素组与对照组相比,无显著性差异。结论汉黄芩素能够减少胶质瘤U87细胞增殖、侵袭,下调ezrin蛋白表达和磷酸化活性,并诱导细胞凋亡。
Objective To investigate the effects of wogonin on proliferation and invasion of glioma U87 cells and its related mechanisms. Methods U87 cells were randomly divided into control group (adding 20μL of culture medium) and (0,50,100) μmol / L wogonin treatment group. The cells were cultured for 48h. The proliferation of U87 cells was detected by MTT assay and the cells were detected by TranswellTM invasion assay Invasion ability, ezrin mRNA expression was detected by real-time fluorescence quantitative PCR, ezrin, Bcl-2, Bax protein expression and ezrin protein phosphorylation levels were detected by Western blot. The terminal deoxynucleotidyl transferase mediated nick end labeling TUNEL method was used to determine the apoptosis index (AI). Results Compared with 0μmol / L Wogonin group and control group, the proliferation inhibition rate of WBC group increased (50,100μmol / L) and the inhibition rate of WBC group increased significantly L Wogonin group. With the increase of wogonin concentration, the average number of transmembrane cells and ezrin mRNA, ezrin protein, ezrin protein phosphorylation level, Bcl-2 protein expression decreased, while Bax protein expression level, AI gradually increased; 0μmol / L Han There was no significant difference between baicalein group and control group. Conclusion Wogonin can reduce the proliferation and invasion of glioma U87 cells, down-regulate ezrin protein expression and phosphorylation activity, and induce apoptosis.