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采用体外细胞培养技术和病毒体内感染模型 ,通过 Elisa、定量 PCR、流式细胞术等方法 ,研究了海洋硫酸多糖 911体外对 HIV- 1复制及体内对 SIV增殖的影响 ,并初步探讨其作用机制。结果发现 911可明显抑制HIV- 1对 MT4 细胞的急性感染和 H9细胞的慢性感染 ,其半数有效浓度 ( EC50 )分别为 4 .4 4 mg· L-1和 0 .32mg· L-1;并可明显降低猴血浆中病毒滴度及 RNA拷贝数 ,对血液中 CD4 +细胞具有一定的保护作用 ,同时可升高血液中病毒抗体的含量 ;并可明显抑制病毒逆转录酶活性 ,对 HIV- 1无明显直接灭活作用 ,但可明显干扰 HIV- 1与细胞的吸附 ,半数有效浓度 ( IC50 )为 36.51μg· L-1。提示 911体内外均可抑制艾滋病毒的增殖 ,为高效的艾滋病毒增殖抑制剂 ,其作用机制与干扰病毒与细胞吸附、抑制病毒逆转录酶活性有关。
Using in vitro cell culture techniques and in vivo viral infection models, the effects of marine sulfated polysaccharide 911 on HIV-1 replication and in vivo proliferation of SIV were studied by Elisa, quantitative PCR, and flow cytometry, and its mechanism of action was preliminarily explored. . As a result, it was found that 911 can significantly inhibit the acute infection of HIV-1 to MT4 cells and the chronic infection of H9 cells with an EC50 of 4.44 mg·L-1 and 0.32 mg·L-1, respectively; It can significantly reduce the virus titer and RNA copy number in monkey plasma, has a certain protective effect on blood CD4 + cells, and can increase the level of virus antibodies in the blood; and can significantly inhibit the activity of the virus reverse transcriptase, for HIV- 1 There was no obvious direct inactivation, but it could significantly interfere with the adsorption of HIV-1 and cells, with an IC50 of 36.51 μg·L-1. It is suggested that 911 can inhibit the proliferation of HIV in vivo and in vitro. It is an effective HIV proliferation inhibitor and its mechanism of action is related to the interference of virus with cell adsorption and inhibition of the activity of reverse transcriptase.