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目的 为了解小肠脂酸结合蛋白基因（ＦＡＢＰ２）多态性与２ 型糖尿病合并冠心病（ＣＨＤ）的关系。方法 ２ 型糖尿病无冠心病组（ＤＭ１）６４ 例，２ 型糖尿病合并冠心病组（ＤＭ２）８２ 例，非糖尿病冠心病组６２ 例，正常对照组６０ 例。应用聚合酶链反应（ＰＣＲ）技术检测２６８ 例对象ＦＡＢＰ２ Ｈｈａ Ｉ位点的限制性片段长度多态性（ＲＦＬＰｓ） 。结果 （１） 武汉地区汉族人存在ＰＡＢＰ２ ＨｈａＩ多态性位点，可产生Ｔｈｒ５４（ － ）和Ｔｈｒ５４（ ＋） 多态片段。（２）２ 型糖尿病合并冠心病患者ＦＡＢＰ２ Ｔｈｒ５４（ ＋） 基因型频率显著高于正常人（Ｐ＜ ０．０５）。（３）ＦＡＢＰ２ Ｔｈｒ５４（ ＋） 基因类型对２ 型糖尿病合并冠心病的参与风险率为２５ ．６ ％ ，比数比为２．１３（９５％ 可信限是１ ．０５ ～４．４５）。（４）２ 型糖尿病合并冠心病组含ＦＡＢＰ２Ｔｈｒ５４（＋ ）基因类型患者与含ＦＡＢＰ２ Ｔｈｒ５４（－ ）基因类型患者比较，空腹胰岛素水平显著增高（ Ｐ＜０．０１），胰岛素敏感指数显著下降（ Ｐ＜ ０．０５） 。（５）２ 型糖尿病合并冠心病组含ＦＡＢＰ２ Ｔｈｒ５４（＋ ）基因类型患者的血甘油三酯水平显著高于含ＦＡＢＰ２ ?
Objective To understand the relationship between the polymorphism of small intestine lipoxygenated binding protein gene (FABP2) and type 2 diabetes with coronary heart disease (CHD). Methods Sixty-two patients with type 2 diabetes without coronary heart disease (DM1), 82 with type 2 diabetes and coronary heart disease (DM2), 62 with non-diabetic coronary heart disease and 60 with normal control group were enrolled in this study. Restriction fragment length polymorphisms (RFLPs) of FHP2 Hha I sites in 268 subjects were detected by polymerase chain reaction (PCR). Results (1) The PABP2 HhaI polymorphism locus existed in Han nationality in Wuhan, which could produce Thr54 (-) and Thr54 (+) polymorphic fragments. (2) The frequency of Frp2 Thr54 (+) genotype in patients with type 2 diabetes mellitus with coronary heart disease was significantly higher than that in normal people (P <0.05). (3) The risk of participation of FABP2 Thr54 (+) genotype in type 2 diabetic patients with coronary heart disease was 25. 6%, and the odds ratio was 2.13 (95% confidence limit was 1.05 ~ 4.45). (4) Compared with patients with FABP2 Thr54 (-) genotype, patients with type 2 diabetes mellitus and coronary heart disease including FABP2Thr54 (-) genotype had significantly higher fasting insulin (P <0.01) and significantly lower insulin sensitivity <0.05). (5) The level of triglyceride in FABP2 Thr54 (+) genotype patients with type 2 diabetes complicated with coronary heart disease was significantly higher than those with FABP2?