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研究CYPⅡE1基因多态性与酒精性脂肪肝发生发展的相关性。从人外周血单个核细胞抽提基因组DNA,采用PCR-RFLP方法对CYPⅡE1基因分型,A型(c1纯合子)、B型(c1/c2杂合子)、C型(c2纯合子)。酒精性脂肪肝组与酗酒无肝病组及健康对照组相比c1基因频率明显降低,c2基因频率明显增高,而酗酒无肝病组与健康对照组相比基因频率无明显差异。c2基因在酒精性脂肪肝的发病中起一定作用。
To investigate the relationship between CYPⅡE1 gene polymorphism and the occurrence and development of alcoholic fatty liver disease. Genomic DNA was extracted from human peripheral blood mononuclear cells. The genotypes of CYPIIE1 were genotyped by PCR-RFLP, type A (c1 homozygotes), type B (c1 / c2 heterozygotes) and type C (c2 homozygotes). The frequency of c1 gene and c2 gene in alcoholic fatty liver group were significantly lower than those in non-alcoholic liver disease group and healthy control group, but there was no significant difference in gene frequency between alcoholic liver disease group and healthy control group. The c2 gene plays a role in the pathogenesis of alcoholic fatty liver disease.