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为了解反复冻融是否对疫苗的感染性滴度产生影响，取浙江省医学科学院普康生物技术公司生产的3批甲肝减毒活疫苗（H2株）进行冻融，每批各12支疫苗，分成4组，每组3支。0组置于4℃，作为原始对照；其它3个组分别进行1次，3次，5次冻融，定为1组，3组，5组。先将疫苗冻入－20℃?
To understand whether repeated freezing and thawing affects the infectious titer of the vaccine, three live attenuated attenuated hepatitis A vaccine strains (H2 strain) produced by Zhejiang Pok Hong Biotechnology Co., Ltd. were frozen and thawed, and each batch of 12 vaccines, Divided into 4 groups of 3 each. Group 0 was placed at 4 ° C as the original control; the other three groups were subjected to 1, 3, and 5 freeze-thaw cycles respectively, and were assigned to groups 1, 3 and 5. First vaccine frozen at -20 ℃?