目的建立恶性疟原虫地理株连续体外培养方法,并采用荧光定量PCR技术分析培养虫株var基因转录类型。方法采用常规RPMI1640培养基和添加AlbumaxⅡ等成分,建立2株恶性疟原虫地理株的体外培养,并采用荧光定量PCR技术检测中晚期虫体var基因转录变化。结果成功体外连续培养2株恶性疟原虫野生株,并建立了荧光定量PCR检测var基因转录的方法,用该方法检测出该虫株var基因优势转录类别。结论建立的疟原虫体外培养方法和var基因转录检测技术可用于我国恶性疟原虫var基因致病作用的分析。 OBJECTIVE: To establish a continuous in vitro culture method for P. falciparum geographic strains and to analyze the var gene transcriptional types of the strains by fluorescence quantitative PCR. Methods Two strains of Plasmodium falciparum were established by conventional RPMI1640 medium supplemented with Albumax Ⅱ, and the var gene transcription was detected by fluorescence quantitative PCR. Results Two wild strains of Plasmodium falciparum were successfully cultured in vitro and a method of quantitative PCR for detecting var gene transcription was established. The predominant var gene transcription type was detected by this method. Conclusion The establishment of Plasmodium in vitro culture methods and var gene transcription detection technology can be used for the analysis of the pathogenesis of var gene in Plasmodium falciparum in our country.