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目的 探讨阿伐他汀 (atorvastatin)对培养的自发性高血压大鼠 (spontaneoushypertensiverats,SHR)和正常血压Wistar大鼠心肌成纤维细胞 (cardiacfibroblasts ,CFs)增殖和胶原合成的影响。方法 采用胰酶消化法培养CFs,用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖 ,流式细胞分析仪 (FCM)技术检测细胞周期 ,ELISA法检测Ⅰ型胶原合成。结果 (1) 10 -7mol/L、10 -6mol/L、10 -5mol/L和 10 -4mol/L阿伐他汀作用后MTT比色法A比值 :SHR组分别为 0 30± 0 0 1、0 2 6± 0 0 1、0 2 4±0 0 1和 0 2 2± 0 0 1,与对照组 (0 33± 0 0 1)比较差异非常显著 (P均 <0 0 1) ;Wistar大鼠组分别为 0 2 8± 0 0 1、0 2 6± 0 0 1、0 2 3± 0 0 1和 0 2 1± 0 0 1,与对照组 (0 30± 0 0 1)相比差异非常显著 (P均 <0 0 1)。 (2 ) 10 -6mol/L阿伐他汀作用 4 8h,SHR和Wistar大鼠CFs的G0 /G1期细胞百分率均较对照组显著增高 (P均 <0 0 1) ,S期细胞百分率、G2 /M期细胞百分率和增殖指数 (proliferationindex,PI)则较对照组显著降低(P均 <0 0 1)。 (3)随着阿伐他汀浓度的增高 ,CFs的Ⅰ型胶原分泌呈明显的递减趋势。 10 -7mol/L、10 -6mol/L、10 -5mol/L和 10 -4mol/L阿伐他汀作?
Objective To investigate the effects of atorvastatin on the proliferation and collagen synthesis of cardiac fibroblasts (CFs) in spontaneous hypertensive rats (SHR) and normotensive Wistar rats. Methods CFs were cultured by trypsin digestion, cell proliferation was measured by MTT colorimetric assay, cell cycle was detected by flow cytometry (FCM), and collagen synthesis was detected by ELISA. Results (1) The ratios of MTT colorimetric method after 10 -7 mol / L, 10 -6 mol / L, 10 -5 mol / L and 10 -4 mol / L atorvastatin: SHR group was 0 30 ± 0 0 1, 0 2 6 ± 0 0 1 0 2 4 ± 0 0 1 and 0 2 2 ± 0 0 1, which were significantly different from the control group (0 33 ± 0 0 1) (all P 0 01) The rats in the control group were 0 28 ± 0 0 1 0 2 6 ± 0 0 1 0 2 3 ± 0 0 1 and 0 2 1 ± 0 0 1, respectively, which was significantly lower than that of the control group (0 30 ± 0 0 1) Very significant (P <0 01). (2) The percentage of cells in G0 / G1 phase in SHR and Wistar rats was significantly higher than that in control group (P <0.01), the percentage of cells in S phase and G2 / M phase cells and proliferation index (proliferationindex, PI) were significantly lower than the control group (P all <0.01). (3) With the increase of atorvastatin concentration, type Ⅰ collagen secretion of CFs showed a significant decreasing trend. 10 -7 mol / L, 10 -6 mol / L, 10 -5 mol / L and 10 -4 mol / L atorvastatin for?