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目的探讨p21codon31单核苷酸多态性与膀胱癌遗传易感性之间的关系。方法应用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测90例膀胱癌患者和110例健康对照个体外周血DNA p21codon31单核苷酸多态性的分布情况。结果膀胱癌与对照组p21codon31的A(arginine)、C(serine)等位基因频率分别为73.89%、22.21%及56.82%、43.18%,两组比较差异有显著性(P<0.05);携带突变(Ser/Arg和Arg/Arg)基因型与野生(Ser/Ser)基因型两组比较,膀胱癌患病风险明显增加(OR,3.375;95%CI,1.553-7.336);膀胱癌按WHO病理分级比较,G1-G2级与G3级比较差异无显著性(P>0.05),按临床分期比较,浅表性癌与浸润性癌突变比较差异也无显著性(P>0.05);G1-G2级膀胱癌OR,2.625(95%CI,1.071-6.432);G2级OR,3.875(95%CI,1.102-13.627);浅表性癌OR,2.688(95%CI,1.038-6.961);浸润性癌OR,3.469(95%CI,1.139-10.564)。结论 p21codon31单核苷酸多态性与中原地区汉族人群膀胱癌的发病风险存在相关性,可能是膀胱癌患病的易感基因。
Objective To investigate the relationship between p21codon31 single nucleotide polymorphism and genetic susceptibility to bladder cancer. Methods The distribution of DNA p21codon31 single nucleotide polymorphisms in peripheral blood of 90 bladder cancer patients and 110 healthy controls was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequencies of the arginine and serine alleles of p21codon31 in bladder cancer and control group were 73.89%, 22.21% and 56.82%, 43.18%, respectively. There were significant differences between the two groups (P <0.05) (OR, 3.375; 95% CI, 1.553-7.336). Compared with the Ser / Ser genotype, Ser / Arg and Arg / Arg genotypes showed a significantly increased risk of bladder cancer There was no significant difference between G1-G2 and G3 (P> 0.05). According to the clinical stage, there was no significant difference between the superficial and invasive cancers (P> 0.05) Grade Bladder cancer OR 2.625 (95% CI, 1.071-6.432); Grade G2 OR 3.875 (95% CI 1.102-13.627); Superficial cancer OR 2.688 (95% CI 1.038-6.961) Cancer OR, 3.469 (95% CI, 1.139-10.564). Conclusions There is a correlation between p21codon31 SNP and the risk of bladder cancer in the Han Chinese in Central Plains, which may be a susceptibility gene to bladder cancer.