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目的 :探讨流式细胞术在严重急性呼吸道综合征 (SARS)诊治中的临床价值。方法 :应用流式细胞仪动态检测SARS患者外周血中淋巴细胞亚群 ,并与健康人进行比较 ,观察其在SARS发病过程中的变化规律。结果 :2例SARS患者的病程约为一个月左右 ,其外周血中CD3 + 、CD3 + CD4+ 、CD3 + CD8+ 绝对计数值最低时仅为 12 0个 /μl、60个 /μl和 5 6个 /μl,随着病情的好转 ,其数值逐渐升高 ,并恢复至正常范围。结论 :应用流式细胞术检测SARS患者外周血中的淋巴细胞亚群 ,可动态监测病情的变化 ,为SARS患者的诊治提供了可靠的依据
Objective: To investigate the clinical value of flow cytometry in the diagnosis and treatment of severe acute respiratory syndrome (SARS). Methods: The peripheral blood lymphocyte subsets of patients with SARS were detected by flow cytometry. The changes of lymphocyte subsets in SARS patients were compared with those in healthy subjects. Results: The course of SARS patients was about one month. The absolute counts of CD3 +, CD3 +, CD4 + and CD3 + CD8 in the peripheral blood were only 120 / μl, 60 / μl and 56 / μ l, as the condition improved, the value gradually increased, and returned to normal range. Conclusion: Flow cytometry can detect lymphocyte subsets in peripheral blood of patients with SARS and can monitor the changes of the disease dynamically, which provides a reliable basis for the diagnosis and treatment of SARS patients