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目的探讨裸小鼠和SCID小鼠两种免疫缺陷动物人胃癌原位移植后肿瘤生长和转移等生物学特性的差异。方法将MKN-45细胞株接种至裸小鼠皮下,成瘤后采用组织学完整的组织块移植于裸小鼠和SCID小鼠胃壁建立原位移植模型,观察所建模型的原位成瘤率、移植瘤生长、侵袭和转移情况。结果①两种免疫缺陷动物的原位成瘤率都为100%;②裸小鼠原位移植瘤平均体积2884±1337mm3,腹腔淋巴结、肝、肺、膈转移率分别为67%、83%、33%和8%;③SCID小鼠原位移植瘤平均体积4582±1326 mm3(P<0.05),肝转移率90%(P>0.05),与裸小鼠较为接近, 腹腔淋巴结转移率90%,肺和膈转移率分别为100%(P<0.01)和60%(P<0.05)。结论证明应用T、B细胞联合免疫缺陷的SCID小鼠较裸小鼠更适用于建立胃癌的原位移植模型。
Objective To investigate the biological characteristics of tumor growth and metastasis after orthotopic transplantation of gastric cancer in nude mice and SCID mice. Methods MKN-45 cells were inoculated subcutaneously into nude mice. After the tumor was established, orthotopically transplanted the nude mice and the SCID mice stomach with histological integrity tissue explants. The in situ tumorigenicity rate of the model was observed , Tumor growth, invasion and metastasis. Results ① The in-situ tumorigenic rates of both immunocompromised animals were 100%. ② The average volume of orthotopic transplanted tumors in nude mice was 2884 ± 1337mm3. The rates of metastasis in abdominal lymph nodes, liver, lung and phrenic were 67%, 83% 33% and 8%, respectively. ③The average volume of SCID mice transplanted in situ was 4582 ± 1326 mm3 (P <0.05) and the liver metastasis rate was 90% (P> 0.05) Lung and phrenic metastasis rates were 100% (P <0.01) and 60% (P <0.05), respectively. The conclusion proves that SCID mice using T and B cells combined with immunodeficiency are more suitable for establishing orthotopic transplantation model of gastric cancer than nude mice.