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一.原理在植物生理试验里,我们时常要用各种试剂比色法与电位测定法来测定溶液中的酸碱度。正如大家所熟知,许多生物化学反应与生理过程都是在水溶液中进行的,构成水分子的H~+与OH~-离子,浓度虽然很小(水在中性时,[H~+]=[OH~-]=10~(-7)M),然而对这些过程的影响却是非常之大。例如说,蛋白质上电荷的正负与多少,就随溶液的H~+浓度而改变。生理溶液中H~+浓度变范围很大,时常会有多少万倍的差别。为了表述的方便,H~+浓度常用
I. Principles In plant physiology experiments, we often use a variety of reagent colorimetry and potentiometry to determine the pH of the solution. As we all know, many biochemical reactions and physiological processes are carried out in aqueous solution, forming water molecules H ~ + and OH ~ - ions, although the concentration is very small (water in the neutral, [H ~ +] = [OH ~ -] = 10 ~ (-7) M), but the impact on these processes is very large. For example, the positive and negative charge on the protein and how much, with the solution of H ~ + concentration change. Physiological solution H ~ + concentration range of a great deal, often how many times the difference. In order to express convenience, H ~ + concentration commonly used