论文部分内容阅读
目的角膜透明性的维持决定于角膜内皮细胞的功能，内皮细胞膜上的钠泵不断地进行着离子转运，从而维持角膜的正常水合状态，这种由离子转运所产生的电位差（跨角膜内皮细胞膜电位）直接反映着角膜内皮细胞的功能状态。本研究通过测定跨角膜内皮细胞膜电位评价角膜保存液的保存效果。方法使用Ｕｓｓｉｎｇ灌流小室分别测定了新鲜兔去上皮角膜片和保存于ＭＫ液、Ｋ液和高钾保存液不同时间的兔去上皮角膜片的跨角膜内皮细胞膜电位。结果新鲜兔角膜跨角膜内皮细胞膜电位值是０．４～０．５±０．１ｍＶ；随着保存时间的延长，３种保存液所保存的角膜片电位值均出现下降，二者呈线性关系。当保存至ｄ１２时，高钾保存液所保存的角膜其电位值最接近新鲜角膜。结论高钾保存液维持角膜内皮细胞活性的作用优于ＭＫ液和Ｋ液。
The purpose of maintaining the transparency of the cornea depends on the function of the corneal endothelial cells, the sodium pump on the endothelial cell membrane continue ion transport, thus maintaining the normal state of corneal hydration, which produced by the ion transport potential difference (across the corneal endothelial cell membrane Potential) directly reflects the functional status of corneal endothelial cells. This study evaluated the preservation of corneal preservation fluid by measuring the transmembrane endothelial cell membrane potential. Methods Ussing perfusion chambers were used to measure the membrane potentials of the rabbit corneal epithelial cells and the epithelial corneal epithelial cells stored in MK fluid, K fluid and high potassium preservative fluid at different times. Results The membrane potential of corneal endothelial cells in fresh rabbit was 0.4-0.5 ± 0.1mV. With the prolongation of storage time, the potential values of corneal membrane stored in all three preservatives decreased, and the linear relationship was found . When preserved to d12, the preservation of corneal potassium preservation solution its potential value closest to the fresh cornea. Conclusion The effect of high potassium preservation solution on maintaining the activity of corneal endothelial cells is better than that of MK solution and K solution.