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目的探讨氟斑牙发生过程中是否存在氧化应激反应。方法 80只SD大鼠按雌雄各半体重均等原则随机分为4组,即饮水氟含量50.00 mg/L(H)组、25.00 mg/L(M)组、12.50 mg/L(L)组和0.08 mg/L(C)组。饲养16周后处死大鼠,测血清及肝、肾组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物(GSHPx)活性。结果 H、M组及部分L组大鼠下切牙釉质出现棕白相间条纹等典型氟斑牙症状。随着染氟剂量的增加,H、M、L组大鼠血清、肝MDA含量较C组有升高趋势(P<0.01或P<0.05),血清及肝、肾SOD、CAT活性较C组有降低趋势(P<0.01或P<0.05),GSH-Px活性变化无统计学意义(P>0.05)。结论氟斑牙发生过程中大鼠体内可能存在氧化系统与抗氧化系统的失衡,氧化应激反应可能是氟中毒早期症状之一。
Objective To investigate whether there is oxidative stress during dental fluorosis. Methods Eighty male Sprague Dawley rats were randomly divided into four groups based on the principle of equal male and female weight: 50.00 mg / L (H), 25.00 mg / L (M), 12.50 mg / L 0.08 mg / L (C) group. After 16 weeks of feeding, the rats were sacrificed and the levels of MDA, SOD, CAT and GSHPx )active. Results The typical dental fluorosis of brown enamel appeared in the enamel of lower incisor of H group, M group and part of L group. With the increase of fluoride dose, the content of MDA in serum and liver of H, M and L groups increased compared with that of C group (P <0.01 or P <0.05), and the activities of SOD and CAT in serum, liver and kidney were higher than those in C group There was no significant difference in GSH-Px activity between the two groups (P <0.01 or P <0.05) (P> 0.05). Conclusion There may be an imbalance between oxidative system and antioxidant system in rats during the occurrence of dental fluorosis. Oxidative stress may be one of the early symptoms of fluorosis.