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目的对犬毛发mtDNA高变区Ⅰ多态性进行检测,并探讨其在法医学中的应用价值。方法 54只家犬(拉布拉多犬7只、史宾格犬7只、德国牧羊犬6只、罗威纳犬4只、藏獒4只、昆明犬4只、杜伯文犬3只、金毛寻猎犬1只、昆明本地犬18只)。采用复合巢式PCR对54只家犬mtDAN HVRⅠ15803～16114区域进行测序分析。结果 54只家犬在mtDNA HVRⅠ15458～16100区域共检测到643个碱基对信息,共检出分属3大单倍群26个多态点;15个单倍型,频率在1.9%～20.4%之间,其中单倍型A11频率最高;个体识别概率为0.898,平均核苷酸差异和平均核苷酸多样度为7.62±3.61和0.011 9±0.006 3。结论采用本文方法,可检测犬mtDNA HVR-Ⅰ多态性,并可利用其较高的个体识别概率为相关案件侦察提供线索或依据。
Objective To detect the polymorphism of mtDNA hypervariable region Ⅰ in dogs and to explore its value in forensic medicine. Methods Fifty-four dogs (7 Labrador dogs, 7 Springer dogs, 6 German shepherds, 4 Rottweilers, 4 Tibetan mastiffs, 4 Kunming dogs, 3 Doberman dogs, Golden Retriever 1 hound, 18 local dogs in Kunming). The mtDAN HVRⅠ15803 ~ 16114 region of 54 domestic dogs were sequenced by multiplex nested PCR. Results A total of 64 polymorphic loci were found in 54 mtDNA HVRⅠ15458 ~ 16100 regions. A total of 26 haplotypes were found in 3 haplogroups, 15 haplotypes (1.9% -20.4%) were detected. Among them, the haplotype A11 had the highest frequency; the individual recognition probability was 0.898, the average nucleotide difference and the average nucleotide diversity were 7.62 ± 3.61 and 0.011 9 ± 0.006 3. Conclusions The mtDNA HVR-Ⅰ polymorphism in dogs can be detected by this method, and its high probability of individual identification can provide clues or evidences for the detection of related cases.