人血清载脂蛋白E显示有明显的多形性,不同的Apo E具有不同的受体结合活性。Apo E的某些表型与高脂蛋白血症和动脉粥样硬化的形成关系密切,Apo E_2纯合子是Ⅱ型高脂蛋白血症必要的诊断条件之一。 国外测定Apo E表型首先利用长达约20小时的超速离心分离人血清VLDL,然后进行等电点聚焦电 Human serum apolipoprotein E shows a marked pleomorphism, and different Apo E have different receptor binding activities. Some phenotypes of Apo E are closely related to the formation of hyperlipoproteinemia and atherosclerosis. Apo E_2 homozygote is one of the necessary diagnostic criteria for type II hyperlipoproteinemia. Foreign Determination of Apo E Phenotype First, human serum VLDL was isolated by ultracentrifugation for up to about 20 hours, followed by isoelectric focusing