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目前国内外纯化鉴定胸腺激素多用等电聚焦、聚丙烯酰胺凝胶电泳等技术。我们在国内首次应用高压液相色谱(HPLC)及荧光扫描分析,本室从猪胸腺提取的胸腺肽—P,探讨其生物活性物质的性质。1.样品:本院生产的胸腺肽—P,批号861106 2.仪器:(1)VARIAN5060高压液相色谱仪。(2)Beckman-DU-8B紫外—可见光光度计。(3)日立850型荧光分光光度计。
Purification and identification of thymus and other current use of isoelectric focusing, polyacrylamide gel electrophoresis and other technologies. For the first time in China, we used high-pressure liquid chromatography (HPLC) and fluorescence scanning analysis to examine the properties of thymosin-P extracted from porcine thymus. 1. Samples: The hospital produced thymosin-P, lot number 861106 2. Instruments: (1) VARIAN5060 high-pressure liquid chromatography. (2) Beckman-DU-8B UV-Vis photometer. (3) Hitachi 850 fluorescence spectrophotometer.