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[目的]采用叶绿素成像技术测定细叶榕(Ficus microcarpaL.)叶片对聚乙二醇模拟的水分胁迫的响应。[方法]人工模拟水分胁迫下检测细叶榕、垂叶榕和极端耐旱的欧洲夹竹桃离体叶片,并用叶绿素荧光成像技术同时提取单片叶多个部位进行检测和分析。[结果]细叶榕、垂叶榕和夹竹桃对脱水响应的结果表明:①叶片各测定部位之间的光合反应中心最大光能转换效率(Fv/Fm)和非光化学猝灭(NPQ)的值较小,而电子传递速率(ETR)、PSII真实光化效率(ΦPSI)I、光化学猝灭(qP)则有较大的差异;②水分胁迫下叶片不同部位的这种离散性更加明显;③抗逆性强的离散程度明显变小。[结论]为叶绿素成像技术进一步应用于植物对干旱胁迫的响应研究奠定了基础。
[Objective] The purpose of this study was to determine the leaf response of Ficus microcarpa L. to PEG-simulated water stress using chlorophyll imaging technique. [Method] The leaves of Ficus microcarpa, Ficus microcarpa and extreme drought-tolerant P. oleander were detected under artificial simulated water stress. Multiple leaf segments were simultaneously extracted and detected by chlorophyll fluorescence imaging technique. [Result] The results of response to dehydration of Ficus microcarpa, Ficus microcarpa and Oleander showed that: (1) The maximum photosynthetic reaction center Fv / Fm and non-photochemical quenching (NPQ) values (ETR), PSII photochemical efficiency (ΦPSI) I, and photochemical quenching (qP) had greater differences. (2) This discrepancy of different parts of leaves was more obvious under water stress; (3) The degree of resistance to strong dispersion is significantly smaller. [Conclusion] The research laid the foundation for the further application of chlorophyll imaging technology in response to drought stress.