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目的 建立人脾原发性恶性淋巴瘤裸小鼠原位移植模型 ,为探讨其发病机理和实验治疗提供工具。方法 将 11例人脾原发性恶性淋巴瘤新鲜组织植入裸鼠脾实质内 ,观察原位移植的成瘤、移植瘤的侵袭和转移及其形态学特征 (光镜、电镜、免疫组织化学 )。结果 筛选出 1株人脾原发性 (非霍奇金B细胞性裂核细胞型 )恶性淋巴瘤裸鼠原位移植模型 (BFNHL HMN 1) ,已传至 41代 ;1株人脾原发性 (非霍奇金B细胞性裂核细胞型 )恶性淋巴瘤裸鼠原位移植肝转移模型 (LM BFNHL HMN 2 ) ,已传至 47代 ;1株人脾原发性 (非霍奇金T免疫母细胞 )恶性淋巴瘤裸鼠原位移植模型 (TINHL HMN 3) ,已传至 37代。共移植裸鼠 6 11只 ,其肿瘤移植生长率、肝转移率和液氮冻存复苏成活率均为10 0 %。BFNHL HMN 1和TINHL HMN 3肿瘤完全限于脾内 ,呈结节状生长 ,或伴有脾门淋巴结累及 ,无腹腔淋巴结和器官转移。LM BFNHL HMN 2肿瘤不仅限于脾脏 ,并有脾门淋巴结及肝转移。原位移植瘤组织经病理学、超微结构观察、流式细胞仪DNA含量测定及染色体核型的分析 ,表明与人脾原发性恶性淋巴瘤细胞相一致。结论 所建立的 3株人脾原发性恶性淋巴瘤裸鼠原位移植模型 ,完整地模拟了人脾原发性恶性淋巴瘤患者的临床过程 ,为研究人脾原发性淋巴瘤的生物学和
Objective To establish a orthotopic transplantation model of human primary malignant lymphoma in spleen and to provide a tool for exploring its pathogenesis and experimental treatment. Methods Fresh tissue of 11 patients with primary malignant spleen lymphoma was implanted into the spleen parenchyma of nude mice. The tumorigenicity, invasion and metastasis of xenograft in nude mice and its morphological characteristics (light microscopy, electron microscopy, immunohistochemistry ). Results A primary in situ transplantion (BFNHL HMN 1) of human spleen lymphoma with malignant lymphoma of human spleen was selected and transmitted to 41 passages. One human primary spleen (Non-Hodgkin B-cell mitosis) malignant lymphoma orthotopic liver transplantation model in nude mice (LM BFNHL HMN 2), has been passed to 47 generations; a human primary spleen (non-Hodgkin’s T Immunoblastoma) Malignant lymphomas Nude mice orthotopic transplantation model (TINHL HMN 3), has been transmitted to 37 generations. A total of 6,11 nude mice were transplanted. The tumor growth rate, liver metastasis rate and survival rate of liquid nitrogen cryopreservation were 100%. BFNHL HMN 1 and TINHL HMN 3 tumors were completely confined to the spleen with nodular growth or with splenic lymph node involvement without celiac lymph nodes and organ metastases. LM BFNHL HMN 2 tumors are not limited to the spleen and have splenic lymph nodes and liver metastases. In situ xenograft tumor tissue pathology, ultrastructure observation, flow cytometry DNA content and chromosome karyotype analysis, and human primary malignant lymphoma cells consistent. Conclusion The established three nude mice model of primary malignant lymphoma of the spleen in situ transplantation model completely simulate the clinical course of patients with primary malignant lymphoma of human spleen, in order to study the biology of primary splenic lymphoma with