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分离和定量测定人类免疫缺陷症病毒(HIV),需要有鉴定感染细胞中病毒或其蛋白或核酸的方法.目前常用的方法有逆转录酶测定、分子杂交或ELISA.这些方法都需要昂贵的设备和材料且费时.作者介绍了一种检测HIV抗原的新方法,称为亲和素-生物素改良的反向被动血凝试验(AB RPHA).它是把人O型Rh阴性红细胞经过处理后,用亲和素包被,然后与生物素化的HIV抗体结合,在1%胎牛血清生理盐水中制成2%悬液,-70℃冻存或加10%葡萄糖冻干.
For the isolation and quantitation of human immunodeficiency virus (HIV), there is a need for methods for identifying viruses or their proteins or nucleic acids in infected cells.Recombinant methods such as reverse transcriptase, molecular hybridization or ELISA are currently used, all of which require expensive equipment And materials and time-consuming.The author introduced a new method for the detection of HIV antigens, called avidin-biotin-modified reverse passive hemagglutination test (AB RPHA) .It is human O-type Rh-negative red blood cells after treatment , Coated with avidin and then conjugated to biotinylated HIV antibody, made into a 2% suspension in 1% fetal bovine serum normal saline, lyophilized at -70 ° C or lyophilized with 10% glucose.