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分别以聚乙二醇(PEG)、聚(丙交酯-乙交酯)(PLGA)和牛血清白蛋白(BSA)为冠、壳和核层材料,采用三层同轴电喷技术制备得到微米颗粒.激光共聚焦显微镜(LSCM)显示,该方法制备得到的微米颗粒呈现核-壳-冠结构.通过脱去该微米颗粒的PEG冠层(模板),得到包载有BSA的纳米颗粒.研究发现,随着壳层PLGA溶液进样速度的减慢,去模板后纳米颗粒的粒径从约146 nm减小到68 nm.BSA在纳米颗粒中的包埋率可高达78.3%,并且其释放没有显著的药物暴释现象.圆二色谱结果表明,同轴电喷过程对BSA二级结构影响很小.因此,利用三层同轴电喷-去模板法可制备得到粒径可调控的蛋白质纳米载体系统,并且该过程中蛋白质的结构基本维持不变.
Polyethylene glycol (PEG), poly (lactide-co-glycolide) (PLGA) and bovine serum albumin (BSA) were used as crown, shell and core materials respectively. Particles.Laser confocal microscopy (LSCM) showed that the microparticles prepared by this method showed a core-shell-crown structure.The nanoparticle loaded with BSA was obtained by removing the PEG canopy (template) It was found that the nanoparticle size decreased from about 146 nm to 68 nm as the PLGA solution injection rate slowed down.BSA encapsulated in nanoparticles could reach 78.3% There was no obvious phenomenon of drug release.The results of circular dichroism showed that the coaxial EFI had little effect on the secondary structure of BSA.Therefore, the three-layer electrospray-to-template method can be used to prepare the protein with adjustable particle size Nano-carrier system, and the process of protein structure remained essentially unchanged.