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目的：观察单纯疱疹病毒Ⅱ型（ＨＳＶ－２）感染极早期对离体人动脉平滑肌细胞（ｈＡＳＭＣ）原癌基因ｃ－ｓｉｓ及ｃ－ｍｙｃ表达的影响。方法：采用不同感染剂量的ＨＳＶ－２吸附单层ｈＡＳＭＣ１小时作为病毒感染细胞模型，应用Ｎｏｒｔｈｅｒｎ杂交方法观察上述原癌基因表达。结果：病毒吸附细胞后０分钟、３０分钟、６０分钟，病毒感染组［５感染复数（ＭＯＩ）和１０ＭＯＩ；ＭＯＩ＝空斑形成单位／细胞数（ＰＦＵ／细胞数）］均比对照组的原癌基因ｃ－ｓｉｓ、ｃ－ｍｙｃ表达增加，且基因表达与感染时间也有关。结论：ＨＳＶ－２感染可以诱导ｈＡＳＭＣ原癌基因ｃ－ｓｉｓ、ｃ－ｍｙｃ表达增加，该作用可能是病毒促进ｈＡＳＭＣ异常增殖进而参与动脉粥样硬化或血管成形术后再狭窄形成的分子机制之一。
OBJECTIVE: To investigate the effect of very early herpes simplex virus type 2 (HSV-2) infection on the expression of proto-oncogene c-sis and c-myc in human aortic smooth muscle cells (hASMC). METHODS: hASMCs were infected with HSV-2 with different infection doses for 1 hour as virus infected cell model. The expression of these proto-oncogenes was observed by Northern hybridization. RESULTS: After virus adsorption at 0, 30, and 60 minutes, the virus infection group [5 MOI and 10 MOI; MOI= plaque forming unit/cell number (PFU/cell count)] were all higher than those of the control group. Oncogene c-sis, c-myc expression increased, and gene expression and infection time are also related. Conclusion: HSV-2 infection can induce the increase of hASMC proto-oncogene c-sis and c-myc expression. This effect may be one of the molecular mechanisms that promote the abnormal proliferation of hASMC and then participate in the formation of restenosis after atherosclerosis or angioplasty. .