该文研究Yes相关蛋白1(Yes-associated protein 1,YAP1)对Eca-109细胞生物学功能的影响。构建针对YAP1基因的sh RNA慢病毒载体,转染Eca-109细胞,采用q PCR和蛋白印迹法检测转染前后Eca-109细胞中YAP1 m RNA和蛋白以及P53蛋白的表达情况;流式细胞仪检测细胞周期、凋亡情况;CCK-8实验检测细胞的增殖情况变化。结果显示,慢病毒转染组细胞内YAP1 m RNA和蛋白表达量低于空白对照组和空病毒转染组,p53基因表达量高于空白对照组和空病毒转染组;Eca-109细胞增值率从第3 d开始低于对照组(P<0.05);慢病毒转染组细胞G1期比例增高(P<0.05),早期凋亡率增加(P<0.05),以上差异均有统计学意义。结果表明,干扰YAP1可诱导Eca-109细胞凋亡,降低Eca-109细胞的增殖能力,且其抗凋亡的作用可能部分与p53基因相关。 This study was aimed to investigate the effect of Yes-associated protein 1 (YAP1) on the biological function of Eca-109 cells. The sh RNA lentiviral vector targeting YAP1 gene was constructed and transfected into Eca-109 cells. The expression of YAP1 m RNA and protein and P53 protein in Eca-109 cells before and after transfection were detected by q PCR and Western blotting. Flow cytometry The cell cycle and apoptosis were detected. The proliferation of cells was detected by CCK-8 assay. The results showed that the expression of YAP1 m RNA and protein in lentiviral transfection group was lower than that in blank control group and empty virus transfection group, the expression of p53 gene was higher than that in blank control group and empty virus transfection group; Eca-109 cell proliferation (P <0.05). The percentage of G1 phase in lentivirus transfection group was increased (P <0.05), and the early apoptosis rate was increased (P <0.05), the above differences were statistically significant . The results showed that interfering with YAP1 could induce apoptosis of Eca-109 cells and reduce the proliferation of Eca-109 cells, and its anti-apoptotic effect may be partly related to p53 gene.