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对３０例启东肝癌病例和１６例对照用竞争抑制放射免疫法检测血清黄曲霉毒素白蛋白加成物，７例血清ＨＢｓＡｇ、抗－ＨＢｃ均阳性的手术病例的石蜡包埋组织用免疫组化法检测ｐ５３蛋白表达。结果表明，病例组黄曲霉毒素白蛋白加成物水平在１．０７～３．４６ｐｍｏｌＡＦＢ１／ｍｇ白蛋白之间，对照组在０．８５～２．９９ｐｍｏｌＡＦＢ１／ｍｇ白蛋白之间，两组比较无显著性差异。６例癌组织、４例癌旁组织ｐ５３蛋白表达阳性，且这６例阳性者黄曲霉毒素白蛋白加成物平均水平为１．８１５±０．６２２ｐｍｏｌＡＦＢ１／ｍｇ白蛋白。提示较长时间内病例和对照黄曲霉毒素暴露的蓄积剂量并无显著性差异。启东肝癌病例ｐ５３蛋白表达率很高，但引起ｐ５３高蛋白表达率的原因尚不能确定
The immunohistochemical method for paraffin-embedded tissues from 7 surgical cases with positive serum HBsAg and anti-HBc was detected in 30 cases of Qidong liver cancer cases and 16 cases of control serum aflatoxin albumin adducts by competitive inhibition radioimmunoassay. Detect p53 protein expression. The results showed that the level of aflatoxin albumin adducts in the case group was between 1.07 and 3.46 pmol AFB1/mg albumin and between 0.85 and 2.99 pmol AFB1/mg albumin in the control group. Significant difference. The expression of p53 protein was positive in 6 cases of cancer tissues and 4 cases of paracancerous tissues, and the average level of aflatoxin albumin adducts in these 6 cases was 1.815±0.622 pmol AFB1/mg albumin. There is no significant difference in the cumulative dose of aflatoxin exposure between the case and the control over a long period of time. The rate of p53 protein expression in Qidong liver cancer cases is very high, but the cause of p53 high protein expression rate cannot be determined