农杆菌介导的转化法是一种天然的遗传工程，具有诸多优越性。 １９９３年 Ｃｈａｎ等首次利用农杆菌法成功地将外源基因转入水稻。随后又有一些实验室进行了农杆菌转化水稻的研究，并获得了较高的转化频率。但大部分的实验都是采用标记基因进行的，有用基因转化的报道则较少。 Agrobacterium-mediated transformation is a natural genetic engineering with many advantages. In 1993 Chan et al. Successfully used the Agrobacterium method to transfer foreign genes into rice successfully. Later, some laboratories carried out Agrobacterium-mediated transformation of rice and obtained higher transformation frequency. However, most of the experiments are performed using marker genes, and fewer reports of useful gene transformations.