论文部分内容阅读
本文旨在研究川芎嗪(tetramethylpyrazine,TMP)拮抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞K~+通道的影响,探讨TMP拮抗链霉素耳毒性的离子通道机制。60只豚鼠随机分为6组,应用听觉脑干反应(auditory brainstem response,ABR)技术检测豚鼠ABR听阈,观测TMP的抗链霉素耳毒作用;并采用全细胞膜片钳技术观察TMP对耳蜗外毛细胞Ca~(2+)敏感K~+电流的影响。结果显示,TMP明显降低链霉素导致的豚鼠ABR听阈升高,提示TMP具有抗链霉素耳毒性作用;TMP能明显增大豚鼠耳蜗外毛细胞Ca~(2+)敏感K~+电流,并呈浓度依赖关系。结果提示,TMP通过增大K~+通道电导而拈抗链霉素耳毒性作用。
This article aims to investigate the effects of tetramethylpyrazine (TMP) on antagonism of streptomycin ototoxicity and K~+ channels in the outer hair cells of guinea pig cochlea, and to explore the mechanism of ion channels that TMP antagonize streptomycin ototoxicity. 60 guinea pigs were randomly divided into 6 groups. Auditory brainstem response (ABR) was used to detect the auditory threshold of ABR in guinea pigs. The effects of TMP on the anti-streptomycin ototoxicity were observed. Whole cell patch clamp technique was used to observe TMP outside the cochlea. Effect of Ca~(2+) Sensitive K~+ Current on Hair Cells. The results showed that TMP significantly decreased the auditory threshold elevation of streptomycin-induced ABR in guinea pigs, suggesting that TMP has anti-streptomycin ototoxicity effects; TMP can significantly increase Ca~(2+) sensitive K~+ currents in guinea pig cochlea outer hair cells. And a concentration-dependent relationship. The results suggest that TMP protects against streptomycin ototoxicity by increasing the conductance of K~+ channels.