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为了寻找一个新的、安全的抗丝虫药物,目前已开始在布鲁丝虫体外培养系统中筛选各种酶的抑制剂和抗丝虫药物。在体外试验中评价药物的指标为:观察成虫的活动性和虫体葡萄糖消耗的最终产物乳酸盐排泄量。本文报道抗疟药甲氟喹对派特丝虫和马来丝虫微丝蚴和成虫的杀灭疗效。从感染多乳鼠的心脏和肺血管内收集二种布鲁丝虫成虫,放置在4～6ml Iscove改良培养基和NCTC135培养基1.1的混和液内,在35℃二氧化碳孵箱内培育。葡萄糖浓度为14mM(1μCi[U~(14)C]-葡萄糖)。从培养基内提取分离物。
In order to find a new and safe anti-filarial medicine, various enzyme inhibitors and anti-filarial drugs have been screened out in the Brucella silkworm in vitro culture system. The indicators of drug evaluation in in vitro tests were: observation of adult activity and lactate excretion of the final product of parasites glucose consumption. This article reports the efficacy of mefloquine, an antimalarial drug, in killing Microfidales and adults of Paratritl and Malayan filariasis. Two species of Brucellosis were harvested from the heart and pulmonary vessels of infected suckling mice and placed in a mixture of 4 to 6 ml Iscove modified media and NCTC135 media 1.1 and incubated in a 35 ° C carbon dioxide incubator. The glucose concentration was 14 mM (1 μCi [U ~ (14) C] -glucose). Isolate was extracted from the medium.