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目的 研究高压氧治疗对脊髓损伤后单核细胞趋化蛋白1(MCP-1)表达及血源性单核细胞浸润的影响.方法 72只SD大鼠随机均分为空白对照组(A组)、脊髓损伤组(B组)和高压氧治疗组(C组).损伤后1、3、7 d取脊髓组织 ,采用RT-PCR检测MCP-1 mRNA表达 ,免疫组织化学法观察MCP-1和ED-1阳性细胞数.结果 与 A 组相比 ,B组损伤后不同时间点 MCP-1表达和ED-1阳性细胞数增加(P<0 .05);而C组MCP-1表达和ED-1阳性细胞数较B组减少(P<0 .05).结论 高压氧治疗能明显抑制脊髓损伤组织中MCP-1的表达 ,减少血源性单核巨噬细胞的浸润 ,减轻局部炎症反应.“,”Objective To investigate the effect of hyperbaric oxygen on monocyte chemotactic protein-1(MCP-1) expression and infiltration of blood-borne monocytes in rats with spinal cord injury (SCI) .Methods Seventy-two SD rats were equally randomized into three groups of A (blank control) ,B(SCI model) and C(hyperbaric oxygen treatment) .The tissues of spinal cord were taken on the 1st ,3rd and 7th day after injury .The mRNA expression of MCP-1 was detected by RT-PCR ,and the positive cells of MCP-1 and ED-1 were observed by immunohistochemistry .Results Compared with group A ,MCP-1 expression and positive cells of ED-1 were increased after injury in group B (P<0 .05) ,which were then reversed by hyperbaric oxygen treatment in group C ( P<0 .05 ) . Conclusion Hyperbaric oxygen treatment can inhibit MCP-1 expression after SCI ,reduce infiltration of blood-borne monocytes and local inflammatory response .