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目的 观察大鼠胚胎神经上皮细胞植入帕金森病大鼠纹状体后的存活、分化情况及治疗作用。 方法 孕 11d大鼠剖腹取胚胎 ,剥离神经管 ,胰酶消化后获取神经管壁上的神经上皮细胞 ,在脑立体定位仪下植入帕金森病大鼠毁损侧纹状体内 ,于移植后不同时间诱发旋转实验 ,观察症状的改善 ,并灌注取脑 ,切片后做酪氨酸羟化酶 (TH)免疫组织化学染色 ,检测胚胎神经上皮细胞的存活及分化状况。 结果 胚胎神经上皮细胞脑内移植后 ,帕金森病鼠的旋转行为有明显改善。移植后 2、4及 8周时可检测到成片或散在的TH 免疫阳性细胞。 结论胚胎神经上皮细胞移植至帕金森病大鼠纹状体后 ,可分化为多巴胺能神经元并能改善旋转症状。
Objective To observe the survival, differentiation and therapeutic effects of rat embryonic rat neuroepithelial cells implanted into the striatum of Parkinson’s disease rats. Methods Embryonic 11-day embryos were taken out of embryos, stripped of neural tube and trypsinized to obtain neuroepithelial cells on the wall of neural tube. The rats were implanted into the lesioned lateral striatum of Parkinson’s disease rats under stereotaxic apparatus. Time-induced rotation experiments were performed to observe the improvement of symptoms. Brain perfusion was performed by perfusion, and tyrosine hydroxylase (TH) immunohistochemical staining was performed to detect the survival and differentiation of embryonic neural epithelial cells. Results After intracerebral transplantation of embryonic neural epithelial cells, the rotation behavior of Parkinson’s disease rats was significantly improved. Thyroid-positive cells could be detected in 2, 4 and 8 weeks after transplantation. Conclusion Embryonic neuroepithelial cells can differentiate into dopaminergic neurons and improve the symptoms of rotation after transplantation into the striatum of Parkinson’s disease rats.