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目的:研究蝎毒抗癌多肽(APⅢ1)对小鼠化疗后致骨髓抑制造血和免疫细胞恢复的影响。方法:应用造血祖细胞培养、流式细胞术等方法,观察APⅢ1对环磷酰胺(CTX)化疗后第14天小鼠骨髓粒单系集落形成单位(CFU-GM)以及CD34、CD117(c-k it)、CD3、CD19等抗原阳性细胞数量的影响,并进行骨髓有核细胞计数和观察小鼠的生存状况。结果:与化疗模型组相比,APⅢ1治疗组的骨髓有核细胞数、CFU-GM集落数、CD34、CD117(c-k it)、CD3、CD19等抗原阳性细胞数量均显著升高,小鼠生存状况有所改善。结论:蝎毒抗癌多肽(APⅢ1)能促进小鼠化疗后致骨髓抑制造血和免疫细胞数量的恢复。
Objective: To study the effect of scorpion venom anti-cancer polypeptide (APIII1) on hematopoietic myelosuppression and immune cell recovery after chemotherapy in mice. METHODS: Cultured hematopoietic progenitor cells and flow cytometry were used to observe the effect of APIII1 on bone marrow mononuclear colony forming units (CFU-GM) and CD34, CD117 on the 14th day after CTX chemotherapy. ), CD3, CD19 and other antigen-positive cells, the number of bone marrow nucleated cells and observe the survival of mice. Results: Compared with the chemotherapy model group, the number of bone marrow nucleated cells, the number of CFU-GM colonies, the number of CD34, CD117 (ck it), CD3, and CD19 antigen positive cells were significantly increased in the APIII1 treatment group, and the survival condition of the mice was significantly increased. it has been improved. Conclusion: The scorpion venom anti-cancer peptide (APIII1) can promote the recovery of hematopoietic and hematopoietic cell numbers induced by chemotherapy in mice.