建立电击法转化稻瘟病菌的操作体系.初步结果表明,运用电击法可以成功地对稻瘟病菌的原生质体实行遗传转化.当使用0.2 cm 电击杯时,最适电击参数为电容25μF,电场强度300 kV·m~(-1),外加电阻800Ω.实验结果同时表明,在电击介质中加入10 g·L~(-1) PEG8000可以显著地提高稻瘟病菌的转化频率.电击法转化具有简便、快速的特点,其转化频率与 PEG 化学融合法相当,可成为 PEG 融合法的一种有效补充. The electroplating method was used to transform the protoplasts of Magnaporthe grisea.When 0.2 cm electric shock cups were used, the most suitable electric shock parameters were the capacitance of 25μF, the electric field strength 300 kV · m -1 and the applied resistance is 800Ω.The experimental results also showed that the addition of 10 g · L -1 PEG8000 to the shock medium could significantly increase the transformation frequency of M. grisea.The electrotransformation method was simple , Rapid characteristics, the frequency of its conversion and PEG chemical fusion method, PEG fusion method can be an effective supplement.