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目的研究并建立见血清药材薄层鉴别与HPLC指纹图谱识别方法。方法采用TLC色谱法,以见血清对照药材为对照,建立薄层色谱鉴别方法;采用HPLC色谱法,建立指纹图谱识别共有模式。结果以石油醚(60~90℃)-乙醚(3∶2)为展开剂展开,能很好分离药材各成分;建立见血清药材HPLC指纹图谱识别方法,并对不同产地的见血清和对照药材进行相似度比较,HPLC指纹图谱标定为19个共有峰,10批不同产地见血清药材相似度均大于0.95。结论见血清药材TLC和HPLC指纹图谱具有重复性好、特征性强、方法简便等特点,可用于见血清药材的有效鉴别。
OBJECTIVE To study and establish the identification method of serum TLC and HPLC fingerprinting. Methods TLC chromatographic method was used to compare the serum reference drugs as control to establish TLC identification method. HPLC chromatographic method was used to establish the common pattern of fingerprint identification. The results of petroleum ether (60 ~ 90 ℃) - ether (3: 2) as the developing agent, can be a good separation of herbs ingredients; to establish the identification of serum herbs HPLC fingerprinting, and to see the serum and control herbs of different origin The similarity was compared by HPLC fingerprinting to identify 19 common peaks. The similarity of serum herbs in 10 batches of different producing areas were all greater than 0.95. Conclusion The TLC and HPLC fingerprints of serum medicine have the characteristics of good repeatability, strong characteristic, simple method and so on, which can be used for the effective identification of serum herbs.