通过单因素试验和正交试验,以相对酶活作为考察指标,研究β-环糊精与α-半乳糖苷酶之间的相互作用。结果表明,不同条件下β-环糊精对α-半乳糖苷酶有不同程度的抑制作用,并且得出了最具抑制效果的反应参数,即在反应体系中,β-环糊精浓度为10 mmol/L,反应时间为90 min,温度为35℃,pH为6.5时β-环糊精对α-半乳糖苷酶的抑制作用最明显,此时相对酶活为19%。 Through single factor test and orthogonal test, the relative enzyme activity was taken as an index to study the interaction between β-cyclodextrin and α-galactosidase. The results showed that β-cyclodextrin inhibited the α-galactosidase to varying degrees under different conditions and obtained the most inhibitory effect of the reaction parameters, that is, in the reaction system, β-cyclodextrin concentration was 10 mmol / L, the reaction time was 90 min, the temperature was 35 ℃, the pH was 6.5, the inhibitory effect of β-cyclodextrin on α-galactosidase was the most obvious, and the relative activity was 19% at this time.