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目的：探讨对应用3种根管倒充填材料玷污层去留对根尖微渗漏的影响，以期为临床应用提供依据。方法：选取64颗单根管牙，常规根管预备后，冷牙胶侧向加压充填根管，根尖切除后进行根管倒预备，将其随机分为实验组与对照组（每组各32颗）。实验组：17％EDTA ＋2％氯亚明去除玷污层，对照组：用5mL 生理盐水冲洗。从2组中随机抽取 2颗牙做扫面电镜检测。各组分别用 MTA、GIC 和 HG 进行倒充填。去除根管上段充填物，将离体牙置于微渗漏模型，于第1、2、4、7、10、15、20和30天检测从冠方向根方漏出的葡萄糖的浓度。结果：MTA 的封闭性在各个时间点均优于 GIC、HG。离体牙同一种处理方法（去除或保留）下不同倒充填材料两两相比，均有统计学差异（P ＜0．05）。同一种倒充填材料在去除与保留玷污层处理中，MTA 、GIC 去除玷污层在各个时间点的葡萄糖渗出浓度均低于保留玷污层，而用 HG 行倒充填则无统计学差异（P ＞0．05）。结论：MTA 的根尖封闭性优于 GIC、HG，应用 MTA、GIC 作为根尖倒充填材料去除玷污层后根尖封闭性更好，而 HG 没有差异。","Objective:Af ect apical microleakage after fil ing material with the smear layer on the application of three root canal.Method:Select 64 single root canal teeth after root canal preparation,cold lateral condensation gut a -percha root canal fil ing,after removal of the apical root canal preparation down.Randomly divided into experimental group and control group (n =30 each).Experimental group:17%EDTA +1% chloramine -T remove the smear layer,In the control group:5ml saline.Randomly selected from two groups do SEMobservation.Each group was randomly divided into 3 groups were MTA,GIC,HG were down fil ing,taking root canal fil ing the upper section.Placing micro -leakage model.First detected in concentrations ranging from 0 days 1,2,4,7,10,15,20,30 crown direction leaked square root of glucose.Results:MTA's closure at each time point were superior GIC,HG.Dif erent back molars same treatment (re-moval or retention)under the fil ing material compared pairwise dif erences were statistical y significant P 0.05).Conclusion:MTA apical closure than GIC,HG,application MTA,GIC as a retrograde fil ing material after removal of the smear lay-er apical sealing bet er,but no dif erence in HG.