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为了用ＤＮＡ 序列分析仪快速、大批量地对大片段ＤＮＡ 进行序列分析研究，我们采用巢式定向渐进性截短缺失突变体构建方法，成功地对一株Ｃ型逆转录病毒基因的大片段ＤＮＡ进行了缺失突变体的构建。实验结果显示，该方法效果好，在大片段ＤＮＡ序列分析研究中具有推广应用价值。文内还对该方法的影响因素进行了讨论。
In order to use DNA sequence analyzer to analyze large fragment DNA rapidly and in large scale, we used nested directional incremental truncated deletion mutants to construct a large fragment DNA of a C retrovirus gene Construction of deletion mutants was performed. The experimental results show that the method is effective and has the value of popularization and application in large DNA sequence analysis. The article also discussed the factors affecting the method.