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目的对云南省不明原因发热患者进行恙虫病东方体检测与分析。方法应用间接免疫荧光方法(IFA)和PCR(nPCR)法对不明原因发热患者分别进行恙虫病东方体(Ot)IgG抗体和sta56基因检测及其基因序列测定分析。结果在79例发热患者中,Ot IgG抗体阳性率48.10%,Ot核酸阳性率3.80%。基因序列分析显示序列12-17与日本O3株同源性最高为99.8%,序列12-9与中国台湾KM05、TW45R等株的同源性最高均为99.8%,序列11-6与中国台湾KM05、TW45R等株的同源性最高均为100%。进化树分析显示序列12-17、12-9和11-6均为Karp型Ot。结论云南省恙虫病患者中存在Karp型Ot感染,其基因进化来源可能复杂。
Objective To detect and analyze Orientia tsutsugamushi in Yunnan patients with unexplained fever. Methods Indirect immunofluorescence (IFA) and PCR (nPCR) were used to detect Ot IgG and sta56 gene of Orientia tsutsugamushi in patients with unexplained fever and their gene sequence analysis respectively. Results In 79 patients with fever, the positive rate of Ot IgG antibody was 48.10% and the positive rate of Ot nucleic acid was 3.80%. Sequence analysis showed that the highest homology of sequence 12-17 with Japanese O3 was 99.8%, the sequence homology of sequence 12-9 with KM05 and TW45R of China Taiwan was 99.8%, and sequence 11-6 was homologous with Taiwan KM05 , TW45R and other strains have the highest homology of 100%. Phylogenetic tree analysis revealed that sequences 12-17, 12-9 and 11-6 are all Karp-type Ot. Conclusion Karp Ot infection exists in patients with tsutsugamushi disease in Yunnan Province, and its gene evolution may be complicated.