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为了研究高羊茅14-3-3基因家族的功能,该研究以拟南芥已报道的13个14-3-3蛋白为基础,通过生物信息分析其潜在的功能,在前期高羊茅转录组与蛋白组学测序获得的14-3-3基因序列为基础,克隆获得4个高羊茅14-3-3基因。通过Clustal W2进行序列比对,发现14-3-3A与14-3-3D,14-3-3B与14-3-3C的同源关系比较近,并且主要结构十分保守。利用荧光定量PCR技术系统分析了14-3-3基因在低氮、干旱、高热以及高盐逆境胁迫下的表达变化发现14-3-3A基因能够应答低氮、干旱、高温以及高盐胁迫,其他成员也能部分应答上述胁迫,但应答机制不一样,可能是通过不同途径调节植物抗逆性,Fa14-3-3基因在进化过程中发生了功能分化。
In order to study the function of the 14-3-3 gene family of tall fescue, the present study, based on 13 14-3-3 proteins reported in Arabidopsis, analyzed its potential function through bioinformatics, Based on the sequence of 14-3-3 gene obtained by sequencing and proteomics, four tall fescue 14-3-3 genes were cloned. Sequence alignment by Clustal W2 showed that the 14-3-3A and 14-3-3D, 14-3-3B and 14-3-3C homologues were relatively close, and the main structure was very conservative. The 14-3-3A gene was able to respond to low nitrogen, drought, high temperature and high salt stress by quantitative real-time PCR analysis of 14-3-3 gene under low nitrogen, drought, high fever and high salt stress. Other members may also partially respond to the above stress, but the mechanism of response is not the same. It may be that plant stress resistance is regulated through different pathways. Fa14-3-3 gene has been functionally differentiated during evolution.