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目的观察HBsAg与HBeAg阴性孕妇的新生儿乙型肝炎病毒(HBV)宫内感染状况。方法选择HBsAg和HBeAg阴性、其他HBV血清标志物阳性孕妇的新生儿作为观察组,同期HBV血清标志物全部阴性孕妇的新生儿作为对照组。采用套式PCR方法检测两组新生儿血清及外周血单个核细胞(PBMC)中的HBV-DNA。结果观察组32例新生儿中,血清HBV-DNA阳性4例,阳性率12.5%(4/32),PBMC中HBV-DNA阳性8例,阳性率25%(8/32),其中血清与PBMC均阳性1例,宫内感染率34.3%(11/32)。对照组21例新生儿血清HBV-DNA和PBMC中HBV-DNA均为阴性。结论HBsAg及HBeAg阴性而其他HBV血清标志物阳性的孕妇也可能发生HBV的垂直传播。
Objective To observe the intrauterine infection of hepatitis B virus (HBV) in neonates with HBsAg and HBeAg negative pregnant women. Methods Neonates with HBsAg and HBeAg negative and other HBV seropositive pregnant women were selected as the observation group. All neonates with negative HBV seropositive for the same period were taken as the control group. The nested PCR method was used to detect HBV-DNA in serum and peripheral blood mononuclear cells (PBMCs) from two groups. Results Among the 32 newborns in the observation group, 4 were positive for HBV-DNA, the positive rate was 12.5% (4/32). The positive rate of HBV-DNA in PBMC was 8% (8/32) 1 were positive, the intrauterine infection rate was 34.3% (11/32). HBV-DNA in 21 neonates of control group and HBV-DNA in PBMC were negative. Conclusion The vertical transmission of HBV may also occur in pregnant women who are negative for HBsAg and HBeAg but have other HBV seropositive markers.