论文部分内容阅读
考查了7种大孔吸附树脂对蛹虫草中虫草素进行吸附纯化效果,以高效液相色谱(HPLC)法测定样液中虫草素含量,筛选出适宜的树脂AB-8,研究了pH值等因素对该树脂静态吸附的影响,以及洗脱剂甲醇体积分数对静态解吸效果的影响,同时还研究了虫草素的动态吸附与解吸曲线。结果表明,AB-8树脂对蛹虫草中虫草素有良好的吸附纯化性能。当上样液pH值为5.0、吸附温度20℃、吸附液质量浓度为0.15 mg.mL-1、上样液量为10倍树脂柱体积及吸附流速为1.8 BV.h-1时,吸附效果最好。洗脱工艺条件为2.5倍树脂柱体积的15%甲醇。
The adsorption and purification of cordycepin in Cordyceps militaris were studied by seven kinds of macroporous adsorption resins. The content of cordycepin in the sample solution was determined by high performance liquid chromatography (HPLC), and the suitable resin AB-8 was screened out. The effects of pH and other factors The influence of the factors on the static adsorption of the resin and the effect of the eluent methanol volume fraction on the static desorption effect were studied. At the same time, the dynamic adsorption and desorption curves of cordycepin were also studied. The results showed that AB-8 resin has good adsorption and purification properties on Cordyceps militaris. When the pH value of the sample solution was 5.0, the adsorption temperature was 20 ℃, the mass concentration of the adsorption solution was 0.15 mg.mL-1, the volume of the sample was 10 times the resin column volume and the adsorption flow rate was 1.8 BV.h-1, the adsorption effect the best. Elution conditions were 2.5% resin column volume of 15% methanol.